2018 | Month: | Volume:5 | Issue:1 | Page:39-42

Role of Cartridge based nucleic acid amplification test (CBNAAT) in new sputum negative pulmonary tuberculosis

Ganesh Chandra Mohapatra, Sumit Bharti,  Mohammed Javed Khan, Smrutiranjan Nayak

Introduction: Diagnosis of pulmonary tuberculosis in sputum negative patients for acid fast bacilli is challenging till today. It is a fact that 50-60% patients of pulmonary tuberculosis are sputum negative microscopically. Smear microscopy is the easiest, commonest and widely employed tool for confirmatory diagnosis of pulmonary tuberculosis, but it has low sensitivity and specificity. Sputum culture for Mycobacterium tuberculosis bacteria can increase the diagnostic yield by 20-40%, but it takes long duration of 2-8 weeks when solid media are used or 10-14 days when radiometric system in liquid media are used. Delayed diagnosis causes increased rate of disease transmission in the community. The role of newly introduced cartridge based nucleic acid amplification test (CBNAAT) in the revised national TB control program (RNTCP) is highly promising with a higher yield of bacteriological diagnosis in sputum negative pulmonary tuberculosis patients with detection of rifampicin resistance within 2 hours only.

Aims and objectives: The current study is to find out efficacy of CBNAAT in diagnosis of new sputum negative pulmonary tuberculosis patients. The detection of rifampicin resistance is also done in those patients. 

Materials and Methods: Sputum samples of 100 sputum negative pulmonary tuberculosis patients were sent to District CBNAAT center in sterilized falcon tube container. The CBNAAT result were analyzed.    Results: Mycobacterium tuberculosis was detected in thirty one patients (31%). Not a single case was detected as resistant to rifampicin.

Conclusion: CBNAAT helps in increased case detection rate in lesser time in new sputum negative pulmonary tuberculosis patients. Rifampicin resistance was not detected in any patient.





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